辣根过氧化物酶
介孔材料
比色法
纳米颗粒
材料科学
硅
化学
双功能
纳米技术
催化作用
色谱法
光电子学
有机化学
酶
作者
Ting Cheng,Weiren Yang,Wenjie Hao,Yongmei Yang,Xiang Li,Taohua Wei,Lulu Tang
标识
DOI:10.1016/j.snb.2021.131312
摘要
The development of methods for highly sensitive and accurate detection of ceruloplasmin (Cp) is of great significance because of the importance of Cp in the diagnosis of Wilson’s disease. Herein, we propose a dual-signal amplification colorimetry-impedance dual-mode high-sensitivity Cp detection immunoassay based on CeO2 encapsulation inside and horseradish peroxidase enrichment outside dendritic mesoporous silicon nanoparticles. This CeO2 nanozyme has high catalytic activity, but its activity is affected by the size of the nanoparticles (as the particle size increases and the activity decreases). Dendritic mesoporous silicon nanoparticles (DMSN) encapsulate CeO2, preventing reduction of its catalytic activity due to self-aggregation, and HRP loading on their surfaces achieves (DMSN-Ce-HA) dual signal amplification to obtain high sensitivity. DMSN-Ce-HA, as a bifunctional probe with excellent catalytic activity is used to catalyze the conversion of a chromogenic substrate for colorimetric readout and 4-chloro-1-naphthol to an insoluble precipitation impedance readout, avoiding the tedious assembly of different probes for dual signal production and reducing the uncontrollable factors of in the experiment process. The proposed colorimetric-impedance dual-mode assay provides more accurate, sensitive, and reliable results than traditional single-signal readout analysis and is therefore a promising candidate for biological analysis.
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