生物发光成像
荧光团
荧光寿命成像显微镜
荧光
生物发光
临床前影像学
双光子激发显微术
光学成像
生物医学工程
体内
分子成像
医学
化学
光学
荧光素酶
生物
物理
生物化学
基因
生物技术
转染
作者
Emilie T. McKinnon,Alfred Moore,Suraj Dixit,Yun Zhu,Ann-Marie Broome
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 283-305
被引量:3
标识
DOI:10.1007/978-1-4939-6646-2_17
摘要
Optical imaging strategies, such as fluorescence and bioluminescence imaging, are non-invasive, in vivo whole body imaging techniques utilized to study cancer. Optical imaging is widely used in preclinical work because of its ease of use and cost-friendliness. It also provides the opportunity to study animals and biological responses longitudinally over time. Important considerations include depth of tissue penetration, photon scattering, absorption and the choice of light emitting probe, all of which affect the resolution (image quality and data information) and the signal to noise ratio of the image. We describe how to use bioluminescence and fluorescence imaging to track a chemotherapeutic delivery nanocarrier conjugated with a fluorophore to determine its localization in vivo.
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