体内
流式细胞术
体外
移植
免疫染色
多巴胺
间充质干细胞
葛根素
纹状体
细胞分化
干细胞
生物
细胞培养
细胞生物学
化学
分子生物学
病理
免疫学
内科学
内分泌学
免疫组织化学
医学
生物化学
生物技术
基因
替代医学
遗传学
作者
Shiying Liu,Xun Qu,Guanghua Jin,Na Feng,Feng Li,Shumei Chen,Fan Hu
标识
DOI:10.1016/j.biopha.2018.07.058
摘要
Parkinson’s disease (PD) is caused by the gradual loss of dopamine-producing cells in the brain. This study evaluated the potential neuroprotective role of puerarin (PR) on dopamine (DA)-producing cells in vitro and in vivo. In vitro, the effects of PR on proliferation and differentiation and DA releases of mesenchymal stem cells (MSCs) were assayed by CCK-8, flow cytometry, real-time PCR and ELISA respectively. Then the differentiated cells were labeled with enhanced green fluorescent protein (EGFP) and administrated into PD animal models induced by 6-OHDA. The proliferation and differentiation of labeled cells were identified by fluorescence microscopy and immunostaining. In vitro, after being treated with different concentrations of PR for 1 week, the TUJ1, TH and DAT protein and mRNA expression and DA releases increased significantly. In vivo, after transplantation of PR-treated DA-producing cells, the symptoms of PD improved significantly from the second week after transplantation; more transplanted cells survived and migrated to wider region along injection line; more transplanted cells proliferated and differentiated into TH+ cells; more DA was detected in the striatum during 6 weeks’ observation. The results suggest that PR promote DA neuron survival, proliferation and differentiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI