血管生成
脐静脉
体内
生物医学工程
血管
组织工程
再生医学
人脐静脉内皮细胞
再生(生物学)
粘附
化学
治疗性血管生成
新生血管
内皮干细胞
细胞生物学
材料科学
体外
细胞
医学
生物
癌症研究
生物化学
有机化学
生物技术
精神科
作者
Rui Yao,Ahmed Yousef F Alkhawtani,Ruoyu Chen,Jie Luan,Mingzhen Xu
出处
期刊:PubMed
日期:2019-01-01
卷期号:5 (2.1): 194-194
被引量:6
标识
DOI:10.18063/ijb.v5i2.1.194
摘要
Rapid reconstruction of functional microvasculature is the urgent challenge of regenerative medicine and ischemia therapy development. The purpose of this study was to provide an alternative solution for obtaining functional blood vessel networks in vivo, through assessing whether hydrogel-based microspheres coated by human umbilical vein endothelial cells (HUVECs) can direct rapid and efficient in vivo angiogenesis without the addition of exogenous growth factors or other supporting cells. Uniform alginate microspheres with adjustable diameter were biofabricated by electro-assisted bioprinting technology. Collagen fibrils were evenly coated on the surface of alginate microspheres through simple self-assembly procedure, and collagen concentration is optimized to achieve the highest HUVECs adhesion and proliferation. Immunofluorescence staining and gene analysis confirmed the formation of the prevascularized tubular structure and significantly enhanced endothelial gene expression. HUVECs-coated hydrogel microspheres with different diameters were subcutaneously injected in immune-deficient mice, which demonstrated rapid blood vessel regeneration and functional anastomosis with host blood vessels within 1 week. Besides, microsphere diameter demonstrated influence on blood vessel density with statistical differences but showed no obvious influence on the area occupied by blood vessels. This study provided a powerful tool for rapid and minimal-invasion angiogenesis of bioprinting constructs and a potential method for vascularized tissue regeneration and ischemia treatment with clinically relevant dimensions.
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