[The Activity Characteristics of ABO Blood Group Glycosyltransferases and the Effect of Plasma Preservation on its activity].

Activities of ABO blood group glycosyltransferases in the plasma of blood donors with different blood groups were detected to discover their normal ranges. In addition, the influence of different plasma storage temperatures and time on the enzyme activity was studied, so as to establish a stable ABO blood group glycosyltransferase activity detection technology system for the auxiliary identification of ABO blood groups.Detect the activities of glycosyltransferase A (GTA) in plasma of type A, AB and O blood donors, and glycosyltransferase B (GTB) in plasma of type B, AB and O blood donors, respectively, to determine the activity range of GTA and GTB in the plasma of normal blood group under this detection technique.The activities of GTA and GTB in plasma of the same ABO blood groups were relatively consistent, while significant difference was found among different ABO blood groups. The activity of GTA was around 27.9±0.3 in plasma of A blood group and 28.3±0.5 in plasma of AB blood group. The activity of GTB in plasma of B blood group was about 24.4±0.5, and that in plasma of AB blood group was about 25.6±0.5. The activities of GTA and GTB in plasma of O blood group were negative. The storage temperature and time of plasma would affect the activities of GTA and GTB. There were no significant changes of the activities of GTA and GTB when the plasma was stored at 4 ℃ for 7 days and -40℃ for 21 days. However, after 28 days of storage at -40 ℃, the activities of GTA and GTB were both decreased significantly.The preservation condition suitable for the detection of ABO glycosyltransferase activity in plasma samples contain short-term storage at 4 ℃ for one week, and cryopreserved at -40 ℃ for no more than three weeks.ABO血型糖基转移酶的活性特征及血浆保存方式对其活性的影响.检测不同血型的无偿献血者血浆中ABO血型糖基转移酶活性，了解其正常范围，并研究血浆的不同保存温度和时间对酶活性的影响，以建立稳定的ABO血型糖基转移酶活性检测技术体系应用于ABO血型的辅助鉴定。.分别检测A型、AB型和O型无偿献血者血浆中A型糖基转移酶（GTA）活性及B型、AB型和O型无偿献血者血浆中B型糖基转移酶（GTB）活性，确定该检测技术下正常血型血浆中的酶活性范围。选取4 ℃和-40 ℃两种保存温度，分别设置不同的保存时间，检测不同保存条件对酶活性是否有影响。.ABO血型糖基转移酶在相同ABO血型的血浆中活性较为一致，在不同ABO血型的血浆中活性有明显差异。GTA在A型血浆中活性为27.9±0.3，在AB型血浆中活性为28.3±0.5；GTB在B型血浆中活性为24.4±0.5，在AB型血浆中活性为25.6±0.5；O型血浆中GTA和GTB均为阴性。血浆的保存温度和保存时间会影响ABO血型糖基转移酶活性。血浆置于4 ℃保存7 d与-40 ℃保存21 d，GTA和GTB活性均无明显改变；-40 ℃保存28 d后，GTA和GTB活性均显著下降（P<0.05）。.适用于不同血型的血浆中ABO糖基转移酶活性检测的血浆标本保存条件为4 ℃短期保存1周，-40 ℃冷冻保存不超过3周。.