安普克
糖原磷酸化酶
糖酵解
AMP活化蛋白激酶
钙
蛋白激酶A
生物学中的钙
化学
细胞生物学
内科学
内分泌学
葡萄糖转运蛋白
糖原
细胞内
激酶
生物
生物化学
新陈代谢
医学
胰岛素
有机化学
作者
Hongju Liao,Lin Zhang,Jiaolong Li,Tieling Xing,Feng Gao
标识
DOI:10.1021/acs.jafc.2c07391
摘要
The current study investigated the effect of preslaughter transport on stress response and meat quality of broilers and explored the underlying mechanisms involved in the regulation of muscle glycolysis through calcium/calmodulin-dependent protein kinase kinase (CaMKK)/AMP-activated protein kinase (AMPK) signaling. Results suggested that transport induced stress responses of broilers and caused PSE-like syndrome of pectoralis major muscle. Preslaughter transport enhanced the mRNA expressions of glycogen phosphorylase and glucose transporters, as well as the activities of glycolytic enzymes, which accelerated the breakdown of glycolytic substrates and the accumulation of lactic acid. In addition, acute stress induced abnormal intracellular calcium homeostasis by disrupting calcium channels on the cell membrane and sarcoplasmic reticulum, which led to the activation of CaMKK and promoted AMPK phosphorylation. This study provides evidence that the intracellular calcium overload and the enhancement of CaMKK/AMPK signaling are related to the accelerated muscle glycolysis of broiler chickens subjected to acute stress.
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