酪氨酸羟化酶
儿茶酚胺能
传出的
内科学
医学
解剖
逆行追踪
缺氧(环境)
内分泌学
自主神经系统
心脏病学
化学
儿茶酚胺
中枢神经系统
心率
血压
免疫组织化学
传入的
有机化学
氧气
作者
Ariege Bizanti,Yuanyuan Zhang,Zulema Toledo,Kohlton Bendowski,Scott W Harden,Anas Mistareehi,Jin Chen,David Gozal,Maci Heal,Richard Christie,Peter Hunter,Julian F. R. Paton,Zixi Cheng
摘要
Abstract Chronic intermittent hypoxia (CIH, a model for sleep apnoea) is a major risk factor for several cardiovascular diseases. Autonomic imbalance (sympathetic overactivity and parasympathetic withdrawal) has emerged as a causal contributor of CIH‐induced cardiovascular disease. Previously, we showed that CIH remodels the parasympathetic pathway. However, whether CIH induces remodelling of the cardiac sympathetic innervation remains unknown. Mice (male, C57BL/6J, 2–3 months) were exposed to either room air (RA, 21% O 2 ) or CIH (alternating 21% and 5.7% O 2 , every 6 min, 10 h day –1 ) for 8–10 weeks. Flat‐mounts of their left and right atria were immunohistochemically labelled for tyrosine hydroxylase (TH, a sympathetic marker). Using a confocal microscope (or fluorescence microscope) and Neurlocudia 360 digitization and tracing system, we scanned both the left and right atria and quantitatively analysed the sympathetic axon density in both groups. The segmentation data was mapped onto a 3D mouse heart scaffold. Our findings indicated that CIH significantly remodelled the TH immunoreactive (‐IR) innervation of the atria by increasing its density at the sinoatrial node, the auricles and the major veins attached to the atria ( P < 0.05, n = 7). Additionally, CIH increased the branching points of TH‐IR axons and decreased the distance between varicosities. Abnormal patterns of TH‐IR axons around intrinsic cardiac ganglia were also found following CIH. We postulate that the increased sympathetic innervation may further amplify the effects of enhanced CIH‐induced central sympathetic drive to the heart. Our work provides an anatomical foundation for the understanding of CIH‐induced autonomic imbalance. image Key points Chronic intermittent hypoxia (CIH, a model for sleep apnoea) causes sympathetic overactivity, cardiovascular remodelling and hypertension. We determined the effect of CIH on sympathetic innervation of the mouse atria. In vivo CIH for 8–10 weeks resulted in an aberrant axonal pattern around the principal neurons within intrinsic cardiac ganglia and an increase in the density, branching point, tortuosity of catecholaminergic axons and atrial wall thickness. Utilizing mapping tool available from NIH (SPARC) Program, the topographical distribution of the catecholaminergic innervation of the atria were integrated into a novel 3D heart scaffold for precise anatomical distribution and holistic quantitative comparison between normal and CIH mice. This work provides a unique neuroanatomical understanding of the pathophysiology of CIH‐induced autonomic remodelling.
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