孟德尔随机化
生物
代谢组
微生物群
银屑病性关节炎
代谢组学
内科学
肿瘤科
遗传学
生物信息学
医学
免疫学
银屑病
遗传变异
基因型
基因
作者
Xiao Yun Xu,Liqiang Wu,Shuyun Wang,Min Yan,Yuanhong Wang,Li Li,Zilin Sun,Ji-Xiang Zhao
标识
DOI:10.3389/fmicb.2024.1287637
摘要
Background Currently, there has been observed a significant alteration in the composition of the gut microbiome (GM) and serum metabolites in patients with psoriatic arthritis (PsA) compared to healthy individuals. However, previous observational studies have shown inconsistent results regarding the alteration of gut microbiota/metabolites. In order to shed light on this matter, we utilized Mendelian randomization to determine the causal effect of GM/metabolites on PsA. Methods We retrieved summary-level data of GM taxa/metabolites and PsA from publicly available GWAS statistics. Causal relationships between GM/metabolites and PsA were determined using a two-sample MR analysis, with the IVW approach serving as the primary analysis method. To ensure the robustness of our findings, we conducted sensitivity analyses, multivariable MR analysis (MVMR), and additional analysis including replication verification analysis, LDSC regression, and Steiger test analysis. Furthermore, we investigated reverse causality through a reverse MR analysis. Finally, we conducted an analysis of expression quantitative trait loci (eQTLs) involved in the metabolic pathway to explore potential molecular mechanisms of metabolism. Results Our findings reveal that eight GM taxa and twenty-three serum metabolites are causally related to PsA ( P < 0.05). Notably, a higher relative abundance of Family Rikenellaceae (OR IVW : 0.622, 95% CI: 0.438–0.883, FDR = 0.045) and elevated serum levels of X-11538 (OR IVW : 0.442, 95% CI: 0.250–0.781, FDR = 0.046) maintain significant causal associations with a reduced risk of PsA, even after adjusting for multiple testing correction and conducting MVMR analysis. These findings suggest that Family Rikenellaceae and X-11538 may have protective effects against PsA. Our sensitivity analysis and additional analysis revealed no significant horizontal pleiotropy, reverse causality, or heterogeneity. The functional enrichment analysis revealed that the eQTLs examined were primarily associated with glycerolipid metabolism and the expression of key metabolic factors influenced by bacterial infections ( Vibrio cholerae and Helicobacter pylori ) as well as the mTOR signaling pathway. Conclusion In conclusion, our study demonstrates that Family Rikenellacea e and X-11538 exhibit a strong and negative causal relationship with PsA. These particular GM taxa and metabolites have the potential to serve as innovative biomarkers, offering valuable insights into the treatment and prevention of PsA. Moreover, bacterial infections and mTOR-mediated activation of metabolic factors may play an important role in this process.
科研通智能强力驱动
Strongly Powered by AbleSci AI