Explore the Constituents and Mechanism of Traditional Chinese Medicine Preparation Zhachong Shisan Pills Based on HPLC-QTOF-MS and Network Pharmacology

Introduction: Th study aims to establish the chromatographic fingerprint of Zhachong Shisan pills (ZSP), and to explore its active components and mechanism of analgesic and anti-inflammatory action. Method: First, the Personal Compound Database and Library (PCDL) of ZSP was constructed through literature mining, and then the chemical composition and fingerprint of methanol extractions from 8 batches of ZSP were studied using High-Performance Liquid Chromatography with Quadrupole Time-Of-Flight tandem Mass Spectrometry (HPLC-QTOF-MS) technology. Furthermore, network pharmacology was used to explore the active compounds, potential targets, and signal pathways of analgesic and anti-inflammatory activity of ZSP. A total of 102 compounds were detected in positive and negative ion mode. Results: Fifty-six characteristic peaks in positive ion mode and 78 characteristic peaks in negative ion mode were confirmed as common peaks in the fingerprint of ZSP. Through network pharmacology research, the effective components, key targets, and signal pathways of ZSP for the treatment of cerebral apoplexy by analgesic and anti-inflammatory effects were all analyzed. Conclusion: This study explained the substance basis of the analgesic and anti-inflammatory effects of Zhachong Shisan Pills, and explores its possible mechanisms, providing ideas for rational clinical medication and in-depth pharmacological research.