ĐÁNH GIÁ SỰ SINH TRƯỞNG VÀ PHÁT TRIỂN CỦA CHỦNG NẤM LINH CHI Ganoderma lingzhi Ga8 THU THẬP TẠI LẠNG SƠN

Collection and evaluation of the natural genetic resources of reishi mushroom is important in the research on medicinal mushrooms. This study aimed to evaluate growth and development characteristics of the newly collected reishi mushroom strain Ga8 in nature, serving basis for applying in production. The reishi Ga8 strain collected in Lang Son province was determined as Ganoderma lingzhi based on morphological characteristics and ITS sequence with 100% similarity in Genebank. The mycelia of the Ga8 strain were cultured on five nutrient media at four different temperature conditions. The results showed that the Ga8 strain grew best on Raper medium with an average mycelial growth rate of 5.56 mm/day. The optimum temperature for mycelial growth was 26°C ± 2. In addition, the Ga8 strain mycelium was also cultured on some master spawn substrates and cultivated. The master spawn substrate consisting of 99% boiled rice and 1% CaCO3 gave the best mycelial growth results, with an average growth rate of 5.72 mm/day. The culture substrate consisted of 87% corn cob, 8% wheat bran, 4% corn powder and 1% CaCO3 yielded highest mushroom biological efficiency of 6.87%