Probiotic strains isolated from natural products alleviate gut inflammation in a human epithelial HT-29 in vitro model

Probiotics are living microorganisms known to benefit human health by maintaining gut microbiota balance and regulating the immune system and inflammation response. Gastrointestinal (GI) inflammation is associated with an unhealthy outcome such as inflammatory bowel disease, irritable bowel syndrome. And supplementation of probiotics can reduce GI inflammation by preventing the loss of tight junction proteins in intestinal epithelial cells, increasing intestinal integrity and reducing lipopolysaccharide (LPS) leakage. Therefore, this study aimed to isolate probiotics strains from natural products and investigate their anti-inflammatory properties. Stability and safety tests were conducted to select probiotic strains. The human colon epithelial HT-29 cell line were used to evaluate the effect of the probiotic strain on relieving intestinal inflammation. After lactic acid bacteria (LAB) treatment, changes in the expression of inflammatory and anti-inflammatory cytokines, tight junction proteins and apoptotic factors were analyzed. Compared to the LPS treatment, the expression of inflammatory and anti-inflammatory cytokines was regulated in the LAB treatment. In addition, the tight junction protein of HT-29 was regulated by LAB treatment and the expression of apoptosis factors increased by LPS was modulated by LAB treatment. As a result, the application of probiotic strains isolated from natural products may reduce intestinal apoptosis and alleviate intestinal inflammation by regulating the expression of cytokines and tight junction protein. Since GI inflammation and gut dysbiosis are related, and GI tract forms an 'axis' with various organs and can affect various aspects of human health, further research is needed to investigate the anti-inflammatory effect using cells from other organs. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.