化学
吸光度
过氧化氢
浊度法
色谱法
儿茶素
丹宁
多酚
没食子酸
浊度
没食子酸表没食子酸酯
核化学
食品科学
有机化学
抗氧化剂
地质学
海洋学
作者
Motokazu Nakayama,Naofumi Shigemune,Takashi Tsugukuni,Harukuni Tokuda,Takahisa Miyamoto
标识
DOI:10.1111/j.1365-2621.2010.02372.x
摘要
Summary We have developed a simple and rapid turbidimetric method to determine catechins based on the fact that many polyphenols produce hydrogen peroxide in an alkaline environment and that hydrogen peroxide oxidises cerium to generate cerium oxide precipitates. Four catechins (epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate) aggregated with these precipitates to form massive precipitates with increased turbidity. The catechins solution (0.18 mL) was mixed with 0.02 mL of 1% CeCl 3 solution, and absorbance (650 nm) was measured immediately after agitation for 3 min using a spectrophotometer. Absorbance was strongly correlated (0.99) with the concentration of each catechin compound. For commercially bottled green tea, the estimated catechin content determined using this turbidimetric method showed better correlation with the content determined by high‐performance liquid chromatography than that determined using ferrous tartrate method, which is the official Japanese method for determining the tannin content of green tea.
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