生物
基因
基因表达
染色质
基因表达调控
酶
线粒体
生物化学
遗传学
细胞生物学
分子生物学
作者
David A. Hall,Heng Zhu,Xiaowei Zhu,Thomas Royce,Mark Gerstein,M Snyder
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2004-10-15
卷期号:306 (5695): 482-484
被引量:220
标识
DOI:10.1126/science.1096773
摘要
Gene expression in eukaryotes is normally believed to be controlled by transcriptional regulators that activate genes encoding structural proteins and enzymes. To identify previously unrecognized DNA binding activities, a yeast proteome microarray was screened with DNA probes; Arg5,6, a well-characterized mitochondrial enzyme involved in arginine biosynthesis, was identified. Chromatin immunoprecipitation experiments revealed that Arg5,6 is associated with specific nuclear and mitochondrial loci in vivo, and Arg5,6 binds to specific fragments in vitro. Deletion of Arg5,6 causes altered transcript levels of both nuclear and mitochondrial target genes. These results indicate that metabolic enzymes can directly regulate eukaryotic gene expression.
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