瘙痒
仓鼠
库鲁
病理
生物
多克隆抗体
内质网
金仓鼠
免疫组织化学
病毒学
抗体
染色
淀粉样蛋白(真菌学)
脂褐素
克雅氏综合征
细胞内
老年斑
阿尔茨海默病
分子生物学
免疫学
医学
朊蛋白
细胞生物学
疾病
作者
Pedro Piccardo,Jiri Safar,Mauro Ceroni,D. C. Gajdusek,Clarence J. Gibbs
出处
期刊:Neurology
[Ovid Technologies (Wolters Kluwer)]
日期:1990-03-01
卷期号:40 (3_part_1): 518-518
被引量:84
标识
DOI:10.1212/wnl.40.3_part_1.518
摘要
We used polyclonal antibodies raised against hamster and mouse PrP27-30 as immunologic probes to study the localization of intracellular and extracellular deposits of prion protein in normal and scrapie-infected mouse and hamster brains and in Creutzfeldt-Jakob disease (CJD)-infected mouse brains. In addition, we examined normal human brain and brain tissues from patients with CJD, kuru, Alzheimer's disease, and idiopathic chronic encephalitis. There was positive staining in the cytoplasm of neurons of normal and scrapie- and CJD-infected mice, and in the neurons of normal and scrapie-infected hamsters. The staining pattern suggests the localization of PrP in an intracellular membrane compartment, most likely the rough endoplasmic reticulum or Golgi apparatus. Antibodies raised against a 15-amino-acid synthetic peptide of the N-terminal of hamster PrP27-30 displayed a similar pattern of staining in mouse brain sections. We observed no intracellular staining in human brain sections obtained at autopsy. Antibodies prepared against mouse and hamster PrP27-30 reacted with amyloid plaques in scrapie-infected mouse and kuru- and CJD-infected human brain sections but not with amyloid plaques in the brain of a patient with Alzheimer's disease.
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