生物催化
化学
流动化学
组合化学
胺化
胺气处理
试剂
级联反应
亚胺
还原胺化
级联
有机化学
反应机理
催化作用
色谱法
作者
Ashley P. Mattey,Grayson J. Ford,Joan Citoler,Christopher Baldwin,Judi Marshall,Ryan B. Palmer,Matthew P. Thompson,Nicholas J. Turner,Sebastian C. Cosgrove,Sabine L. Flitsch
标识
DOI:10.1002/anie.202103805
摘要
A key aim of biocatalysis is to mimic the ability of eukaryotic cells to carry out multistep cascades in a controlled and selective way. As biocatalytic cascades get more complex, reactions become unattainable under typical batch conditions. Here a number of continuous flow systems were used to overcome batch incompatibility, thus allowing for successful biocatalytic cascades. As proof-of-principle, reactive carbonyl intermediates were generated in situ using alcohol oxidases, then passed directly to a series of packed-bed modules containing different aminating biocatalysts which accordingly produced a range of structurally distinct amines. The method was expanded to employ a batch incompatible sequential amination cascade via an oxidase/transaminase/imine reductase sequence, introducing different amine reagents at each step without cross-reactivity. The combined approaches allowed for the biocatalytic synthesis of the natural product 4O-methylnorbelladine.
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