内质网
高尔基体
细胞生物学
GTP酶
拟南芥
复印机
生物
ADP核糖基化因子
GTPase激活蛋白
分泌途径
拟南芥
生物化学
G蛋白
信号转导
基因
突变体
作者
Myung Ki Min,Soo Jin Kim,Yansong Miao,Juyeon Shin,Liwen Jiang,Inhwan Hwang
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2007-02-16
卷期号:143 (4): 1601-1614
被引量:68
标识
DOI:10.1104/pp.106.095091
摘要
ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms. Here, we investigated the biological role of AGD7, an Arf GAP homolog, in Arabidopsis (Arabidopsis thaliana). We show that AGD7 bears a highly conserved N-terminal region and a unique C-terminal region, interacts with Arf1 both in vitro and in vivo, and stimulates Arf1 GTPase activity in a phosphatidic acid-dependent manner in vitro. In plant cells, AGD7 localized to the Golgi complex, where its overexpression was found to inhibit the Golgi localization of γ-subunit of coat proteins and promote the relocation of Golgi proteins into the endoplasmic reticulum in both protoplasts and transgenic plants. Furthermore, overexpression of AGD7 inhibited anterograde trafficking of proteins from the endoplasmic reticulum. We propose that AGD7 functions as a GAP for Arf1 in the Golgi complex and plays a critical role in protein trafficking by controlling Arf1 activity.
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