离体
PLGA公司
淋巴系统
微熔池
化学
病理
共焦显微镜
原位
体内
淋巴
上皮
生物
生物物理学
解剖
细胞生物学
体外
医学
生物化学
有机化学
生物技术
作者
Anne-Marie Torché,H Jouan,Pascal Le Corre,Emmanuel Albina,Roselyne Primault,André Jestin,Roger Le Verge
标识
DOI:10.1016/s0378-5173(00)00364-1
摘要
We investigated the ability of pig ileal Peyer’s patch segments to transport intestinal poly (d,l-lactide-co-glycolide) microspheres (PLGA MS) from intestinal lumen across the mucosae using in situ and ex vivo segments with confocal laser scanning microscopy (CLSM) and transmission electronic microscopy (TEM). From a global aspect, CLSM suggested that PLGA MS were translocated by M cells labelled with a FITC-conjugated anti-cytokeratin peptide 18, and transported through the follicle-associated epithelium (FAE) in the dome area in both types of experiments. At the ultrastructural level, TEM showed the traffic of PLGA MS throughout M cells, their transport into the basolateral invaginations of the M cells and their subsequent migration into the dome area and the follicular area in contact with macrophages and lymphatic vessels. Although in situ experiments allowed following the migration of PLGA MS until mesenteric lymph nodes, an ex vivo model could be used as a useful tool to study the targeting ability of PLGA MS formulations to the gut-associated lymphoid tissue (GALT).
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