海参
皂甙
三萜
糖苷
化学
色谱法
硫酸化
萃取(化学)
立体化学
生物
生物化学
生态学
医学
病理
替代医学
作者
Yadollah Bahrami,Wei Zhang,Tim Chataway,Christopher M. M. Franco
出处
期刊:Marine Drugs
[MDPI AG]
日期:2014-08-08
卷期号:12 (8): 4439-4473
被引量:39
摘要
Sea cucumbers are prolific producers of a wide range of bioactive compounds. This study aimed to purify and characterize one class of compound, the saponins, from the viscera of the Australian sea cucumber Holothuria lessoni. The saponins were obtained by ethanolic extraction of the viscera and enriched by a liquid-liquid partition process and adsorption column chromatography. A high performance centrifugal partition chromatography (HPCPC) was applied to the saponin-enriched mixture to obtain saponins with high purity. The resultant purified saponins were profiled using MALDI-MS/MS and ESI-MS/MS which revealed the structure of isomeric saponins to contain multiple aglycones and/or sugar residues. We have elucidated the structure of five novel saponins, Holothurins D/E and Holothurinosides X/Y/Z, along with seven reported triterpene glycosides, including sulfated and non-sulfated saponins containing a range of aglycones and sugar moieties, from the viscera of H. lessoni. The abundance of novel compounds from this species holds promise for biotechnological applications.
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