Silver nanoparticles enhanced glucose oxidase electrodes, which are based on polyvinyl butyral and chitosan, were fabricated and investigated. The sensitivity and maximum current i max of the chitosan‐based electrode are 1.06 mA · M−1 and 25.64 µA, respectively. However, the sensitivity and i max of the polyvinyl butyral‐based electrode are 0.34 mA · M−1 and 7.22 µA. The chitosan‐based electrode has a sensitivity 3.1 times higher and a maximum current 3.6 times larger than those of the polyvinyl butyral‐based electrode. Therefore, it is more sensitive and stable than the polyvinyl butyral‐based electrode.