丝氨酸
五肽重复序列
脂肪酶
定点突变
酯酶
活动站点
生物化学
丝氨酸水解酶
单酰甘油脂肪酶
突变
化学
突变
生物
突变体
酶
脂蛋白脂酶
氨基酸
甘油三酯酶
肽序列
催化三位一体
结合位点
共识序列
分子生物学
蛋白质一级结构
序列(生物学)
鉴定(生物学)
作者
Cecilia Holm,Richard C. Davis,Torben Østerlund,Michael C. Schotz,Gudrun Fredrikson
出处
期刊:FEBS Letters
[Wiley]
日期:1994-05-16
卷期号:344 (2-3): 234-238
被引量:63
标识
DOI:10.1016/0014-5793(94)00403-x
摘要
The consensus pentapeptide GXSXG is found in virtually all lipases/esterases and generally contains the active site serine. The primary sequence of hormone‐sensitive lipase contains a single copy of this pentapeptide, surrounding Ser‐423. We have analyzed the catalytic role of Ser‐423 by site‐directed mutagenesis and expression of the mutant hormone‐sensitive lipase in COS cells. Substitution of Ser‐423 by several different amino acids resulted in the complete abolition of both lipase and esterase activity, whereas mutation of other conserved serine residues had no effect on the catalytic activity. These results strongly suggest that Ser‐423 is the active site serine of hormone‐sensitive lipase.
科研通智能强力驱动
Strongly Powered by AbleSci AI