代谢组
代谢途径
基因表达谱
计算生物学
RNA序列
蛋白质组学
小桶
遗传学
次生代谢
MYB公司
仿形(计算机编程)
作者
Wei Chang,Huina Zhao,Shizhou Yu,Jing Yu,Kai Cai,Wei Sun,Xumei Liu,Xiaodong Li,Mengna Yu,Shahzad Ali,Kai Zhang,Cunmin Qu,Bo Lei,Kun Lu
出处
期刊:Genomics
[Elsevier]
日期:2020-07-07
卷期号:112 (6): 4009-4022
被引量:4
标识
DOI:10.1016/j.ygeno.2020.07.005
摘要
Although the leaf is the most important photosynthetic organ in most plants, many of the molecular mechanisms underlying leaf developmental dynamics remain to be explored. To better understand the transcriptional regulatory mechanisms involved in leaf development, we conducted comparative transcriptomic and metabolomic analysis of leaves from seven positions on tobacco (Nicotiana tabacum) plants. A total of 35,622 unique differentially expressed genes and 79 metabolites were identified. A time-series expression analysis detected two interesting transcriptional profiles, one comprising 10,197 genes that displayed continual up-regulation during leaf development and another comprising 4696 genes that displayed continual down-regulation. Combining these data with co-expression network results identified four important regulatory networks involved in photorespiration and the tricarboxylic acid cycle; these networks may regulate carbon/nitrogen balance during leaf development. We also found that the transcription factor NtGATA5 acts as a hub associated with C and N metabolism and chloroplast development during leaf development through regulation of phytohormones. Furthermore, we investigated the transcriptional dynamics of genes involved in the auxin, cytokinin, and jasmonic acid biosynthesis and signaling pathways during tobacco leaf development. Overall, our study greatly expands the understanding of the regulatory network controlling developmental dynamics in plant leaves.
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