Study on HPLC Fingerprint of Alpinia katsumadai Hayata

Abstract Objective: to establish HPLC fingerprint of Alpinia katsumadai Hayata. Method: 10 batches of Alpinia katsumadai Hayata was analyzed by HPLC that the methods were used Diasonsil C18 chromatographic column, with the mobile phase of acetonitrile-0.1% phosphoric acid solution, the flow rate was 1mL/min, the wavelength was 220nm, and column temperature was 30°C. Similarity of 10 batches of Alpinia katsumadai Hayata was evaluated by similarity evaluation software. Result: The HPLC fingerprint of Alpinia katsumadai Hayata was established, and 19 common peaks were determined, the similarity was between 0.995-0.822. It shows that the difference between the 10 batches of Alpinia Katsumadai Hayata was not significant. Conclusion: The analysis method is fast in operation, good in separation, strong in stability and good in repeatability, which can provide reliable basis for quality control of Alpinia katsumadai Hayata.