壳聚糖
甲壳素
傅里叶变换红外光谱
贻贝
核化学
生物高聚物
化学
抗菌活性
萃取(化学)
聚合物
色谱法
化学工程
生物
渔业
细菌
生物化学
有机化学
工程类
遗传学
作者
Rahul Varma,Sugumar Vasudevan
出处
期刊:ACS omega
[American Chemical Society]
日期:2020-08-06
卷期号:5 (32): 20224-20230
被引量:137
标识
DOI:10.1021/acsomega.0c01903
摘要
Chitin and chitosan have been proved to have enormous applications in biomedical, pharmaceutical, and industrial fields. The horse mussel, Modiolus modiolus, a refuse of the fishery industries at Thondi, is a reserve of rich chitin. The aim of this work is to extract chitosan from the horse mussel and its further characterization using Fourier transform infrared spectroscopy (FTIR), micro-Raman spectroscopy, X-ray diffraction (XRD), and elemental analysis. The result of FTIR studies revealed different functional groups of organic compounds such as out-of-plane bending (564 cm-1), C-O-C stretching (711 cm-1), and CH2 stretching (1174 cm-1) in chitosan. The degree of acetylation of the extracted chitosan was observed to be 57.43%, which makes it suitable as a biopolymer for biomedical applications. Prominent peaks observed with micro-Raman studies were at 484 cm-1 (14,264 counts/s), 2138 cm-1 (45,061 counts/s), and 2447 cm-1 (45,636 counts/s). XRD studies showed the crystalline nature of the polymer, and the maximum peak was observed at 20.04°. Elemental analysis showed a considerable decrease in the percentage of nitrogen and carbon upon the conversion of chitin to chitosan, while chitosan had a higher percentage of hydrogen and sulfur. The antibacterial activities of chitosan from the horse mussel were found to be efficient at a 200 μg/mL concentration against all the bacterial strains tested with a comparatively higher antibacterial activity against Escherichia coli (9 mm) and Bacillus subtilis (8 mm).
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