化学
糖基转移酶
糖基化
韧皮部
甘草
立体化学
氢键
糖
糖苷
晶体结构
基质(水族馆)
生物化学
分子
酶
有机化学
替代医学
病理
地质学
海洋学
医学
作者
Ming Zhang,F.D. Li,K. Li,Z.L. Wang,Y.X. Wang,Junbin He,Huifei Su,Z.Y. Zhang,Chongwei Chi,Xiaomeng Shi,Cai‐Hong Yun,Z.M. Liu,L.R. Zhang,Dong Yang,Ming Ma,Xue Qiao,M. Ye
摘要
A highly efficient di-C-glycosyltransferase GgCGT was discovered from the medicinal plant Glycyrrhiza glabra. GgCGT catalyzes a two-step di-C-glycosylation of flopropione-containing substrates with conversion rates of >98%. To elucidate the catalytic mechanisms of GgCGT, we solved its crystal structures in complex with UDP-Glc, UDP-Gal, UDP/phloretin, and UDP/nothofagin, respectively. Structural analysis revealed that the sugar donor selectivity was controlled by the hydrogen-bond interactions of sugar hydroxyl groups with D390 and other key residues. The di-C-glycosylation capability of GgCGT was attributed to a spacious substrate-binding tunnel, and the G389K mutation could switch di- to mono-C-glycosylation. GgCGT is the first di-C-glycosyltransferase with a crystal structure, and the first C-glycosyltransferase with a complex structure containing a sugar acceptor. This work could benefit the development of efficient biocatalysts to synthesize C-glycosides with medicinal potential.
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