Holographic Proteomics: A Review of Digital Holographic MicroscopyApplications in Spatial Proteomics

Holoproteomics is a state-of-the-art advancement in spatial proteomics, enabling comprehensive spatial analysis of proteins in tissue microenvironments by combining Digital Holographic Microscopy (DHM) imaging and high-precision laser capture microdissection (LCM) techniques. DHM is an advanced technology that utilizes optical interference principles for non-invasive imaging, providing high-resolution three-dimensional visualization of cells and macromolecules without requiring markers. In proteomics, DHM provides crucial technical support for investigating protein interactions and enables high-precision tracking and analysis of dynamic protein changes. In this review, we systematically survey peer-reviewed literature published in the past five years, with a focus on experimental and clinical studies applying DHM to proteomic analyses. Based on the significant advantages of this technology, we introduce the concept of “Holographic proteomics” as an emerging research field with promising future directions.