化学
动力学分辨率
基质(水族馆)
水解
反应速率
扁桃酸
色谱法
对映体过量
传质
生物催化
动力学
对映体
有机化学
催化作用
对映选择合成
反应机理
海洋学
物理
量子力学
地质学
作者
Shuzhan Wang,Tingting Shi,Zheng Fang,Chengkou Liu,Wei He,Ning Zhu,Yujing Hu,Xin Li,Kai Guo
标识
DOI:10.1007/s41981-022-00219-z
摘要
During the kinetic resolution process, increased substrate conversion often compromised the optical purity of products. A strategy integrated biotechnology and microtechnology was developed to kinetic resolution of mandelic acids. The effects of temperature, pH, solvent, reaction time, flow rate on the hydrolysis were conducted. Under optimal conditions, the MCR reaction afforded 95% eep, whereas the batch reaction yielded 65% eep. The kinetics of hydrolysis was also investigated. The value for Km and Vmax in batch was 12 mM and 11 mM s−1, while that in MCR was 8 mM, 45 mM s−1. This means more substrates can be transferred to the active site on the enzyme in MCR at the same concentration. This is a benefit from the excellent mass transfer limitations in MCR. Furthermore, the apparent Vmax was significantly increased, closer to the intrinsic rate of reaction. Finally, the strong mass transfer in MCR leads to an expanded rate difference between (R) enantiomers and (S) enantiomers, improving the eep and E-values.
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