摘要
de Abreu, R. C., Ramos, C. V., Becher, C., Lino, M., Jesus, C., da CostaMartins, P.A., Martins, P. A. T., Moreno, M. J., Fernandes, H., & Ferreira, L. (2021). Exogenous loading of miRNAs into small extracellular vesicles. Journal of Extracellular Vesicle, 10(10), e12111. https://doi.org/10.1002/jev2.12111 Reference citations are inadvertently placed in the caption of Figure 1, whereas there should not be any reference citation. The correct caption for Figure 1 is: Figure 1 Schematic representation of the different methods used to modulate sEVs with a Cy3-labelled miRNA mimic and the follow-up assays performed to validate the modulation and assess the bioactivity of the modulated sEVs. Five different methodologies have been used to load miRNAs into sEVs: transfection by Exo-Fect or cholesterol-modified miRNA and membrane permeabilization by a detergent (saponin), electroporation or heat shock (1). The modified sEVs were then characterized regarding their loading efficiency by fluorescence and qRT-PCR analyses (2), bioactivity in the HEK-293T reporter cell line (3), cell toxicity using a cell viability assay (4) and capacity to transfect cells (5).