Integrated dual-channel electrochemical immunosensor for early diagnosis and monitoring of periodontitis by detecting multiple biomarkers in saliva

Periodontitis, as the sixth prevalence chronic inflammation worldwide, has inconspicuous and often-overlooked symptoms at early stage, eventually leading to permanent damage to the teeth and supporting tissues. The timely and accurate diagnosis of periodontitis and monitoring its progress appear to be particularly important for clinical treatment. Herein, a dual-channel electrochemical immunosensor was developed for the synchronized detection of two periodontitis-related biomarkers in saliva: interleukin-1β (IL-1β) and matrix metalloproteinase-8 (MMP-8). Owing to its miniaturization, detachability, and portability, this sensor has the potential to detect multiple biomarkers in a point-of-care manner for the early diagnosis and monitoring of periodontitis. The nanocomposites consisted of iridium oxide nanotubes and two-dimensional MXene nanosheets enhance the electrochemical performance of the sensor, achieving excellent sensitivity with wide detection ranges of 0.1-100 and 1-200 ng mL-1, low limits of detection of 0.014 and 0.13 ng mL-1, and relatively high correlation coefficients of 0.9911 and 0.9990 for IL-1β and MMP-8, respectively. Furthermore, this device possesses excellent selectivity in complex samples without cross-talk, as well as high recovery and accuracy in spiked artificial saliva. Importantly, the dual-channel device achieves higher diagnostic accuracy for different stages of periodontitis when MMP-8 and IL-1β were simultaneously monitored within clinicopathological saliva. This work proposes a considerable potential for early diagnosis and severity distinguishment of periodontitis in a point-of-care manner, which would be beneficial for progression prediction, treatment guidance, and prognosis assessment of periodontitis.