A target induced self-powered catalyzed hairpin assembly coupled with DNA motor amplification for miRNA detection in colorectal cancer

A target induced self-powered catalyzed hairpin assembly coupled with DNA motor amplification strategy was presented for miRNA-92a detection. The miRNA is used to open the hairpin and initiate the catalyzed hairpin assembly (CHA). The product of CHA can catalyze the CHA reaction and cause the recovery of fluorescent intensity. The limit of detection (LOD) could reach to 0.04 fM with good linear range from 0.2 fM to 500 fM. Importantly, the time of two level amplification reaction was greatly shortened to 130 min by the high efficiency of self-powered CHA strategy. The amplification efficiency of self-powered CHA strategy was investigated. It also showed great selectivity and specificity for other miRNA sequences. In addition, it exhibited satisfactory results for practical application.