Synergistic protection of combined Aronia melanocarpa Elliot anthocyanins with Aloe Polysaccharides inhibits alcoholic liver injury in mice

In this study, network pharmacology was used to predict the possible targets of Aronia melanocarpa Elliot anthocyanins (AMA) and Aloe Polysaccharides (APs) on alcoholic liver disease (ALD), and animal experiments were conducted to explore the mitigation effect and mechanism of AMA combined with APs on ALD, in order to provide experimental basis for the prevention and treatment of ALD. The network pharmacology analysis showed that 115 core targets were obtained by the intersection of active components of AMA and APs and the targets related to ALD, the core targets were mainly related to the positive regulation of oxidative stress and apoptosis. Animal experiments showed that AMA and APs could inhibit the weight loss of ALD mice; reduce the liver index of mice; reduce the levels of AST, ALT, TC, TG in serum of mice; increase the content of GSH-PX in liver tissue, reduce the content of ALDH in liver tissue; reduce the expression of inflammatory factors; improver the morphology of liver tissue in mice; increase the expression of activated PI3K/AKT signaling pathway, promote the expression of Nrf2 and HO-1, and improve the symptoms of ALD. However, the combination therapy produced better results. This study found that compared with single administration, AMA combined with APs administration had better remission effect on ALD, which was expected to provide a theoretical basis for the development and utilization of AMA and APs.