高分子拥挤
细胞外基质
结缔组织
组织工程
高分子
生物物理学
体外
细胞培养
细胞生物学
Ⅰ型胶原
前胶原肽酶
化学
材料科学
生物医学工程
生物化学
生物
分子生物学
医学
遗传学
内分泌学
作者
Joseph Krebs,Samuel Stealey,Alyssa Brown,Aaron J. Krohn,Silviya Petrova Zustiak,Natasha Case
出处
期刊:Gels
[MDPI AG]
日期:2023-09-01
卷期号:9 (9): 705-705
被引量:2
摘要
Connective tissue models grown from cell monolayers can be instrumental in a variety of biomedical fields such as drug screening, wound healing, and regenerative engineering. However, while connective tissues contain abundant fibrillar collagen, achieving a sufficient assembly and retention of fibrillar collagen in vitro is challenging. Unlike the dilute cell culture environment, the body's environment is characterized by a high density of soluble macromolecules (crowding) and macromolecular networks (confinement), which contribute to extracellular matrix (ECM) assembly in vivo. Consequently, macromolecular crowding (MMC) has been successfully used to enhance the processing of type I procollagen, leading to significant increases in fibrillar collagen assembly and accumulation during in vitro culture of a variety of cell types. In this study, we developed a combination approach using a carrageenan hydrogel, which released soluble macromolecules and served as a confinement barrier. We first evaluated the local carrageenan release and then confirmed the effectiveness of this combination approach on collagen accumulation by the human MG-63 bone cell line. Additionally, computational modeling of oxygen and glucose transport within the culture system showed no negative effects of the hydrogel and its releasates on cell viability.
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