Enhanced osteogenesis and inflammation suppression in 3D printed n-HA/PA66 composite scaffolds with PTH(1-34)-loaded nPDA coatings

In current clinical applications, spinal fusion predominantly are mostly inertness biomaterials with dense structures, which restrict the interface for new bone growth and impede the formation of interlocking structures, thereby caused failed bone integration. To address these challenges, this research proposed a novel 3D printed nano-hydroxyapatite/polyamide66 (n-HA/PA66) composited biological scaffold. Fused Deposition Modeling (FDM) technology was used to fabricate optimized porous architecture scaffold with balanced mechanical properties. A coating of polydopamine nanoparticles (nPDA) loaded with Parathyroid Hormone (PTH) (1-34) was then prepared on the scaffold surface, it effectively mitigated the inflammatory response and enhancing bone integration. In vitro results indicated a sustained release of PTH (1-34) would promote the osteogenic ability of scaffold. In vivo results demonstrated that the composited biological scaffold exhibited good ability to induce bone tissue regeneration which is beneficial for spinal fusion applications.