石墨烯
电化学发光
纳米花
血红素
检出限
生物传感器
线性范围
分析物
纳米化学
适体
化学
氧化物
胶体金
材料科学
纳米技术
组合化学
色谱法
核化学
纳米颗粒
纳米结构
生物化学
分子生物学
酶
有机化学
生物
血红素
作者
Gengjun Liu,Xiaohan Guan,Binxiao Li,Hong Zhou,Na Kong,Haiyan Wang
标识
DOI:10.1007/s00604-022-05387-2
摘要
An ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed for the detection of prostate specific antigen (PSA) using glucose oxidase-decorated hemin-graphene oxide-gold nanoflowers ternary nanocomposites as probes. Graphene oxide was first modified with hemin and then with gold nanoflowers through an in situ growth method, which has significantly boosted the catalytic activity of this graphene oxide-based peroxidase mimetics. The biocatalytical activity of this ECL immunosensor was thoroughly investigated to achieve selective recognition of the analyte molecules (PSA) by specific binding between antigens and antibodies. The limit of detection was calculated to be 0.32 pg mL-1 with a signal-to-noise ratio of 3. A broad linear range from 7.5 × 10-4 to 2.5 ng mL-1 was obtained. Such step-by-step assembled biosensor showed controlled nanostructure and exhibited promising application in analysis of human serum samples with a recovery range of 90.6-111.8% and a RSD range of 3.9-5.5%.
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