Janus nanozyme based satellite structure immunosandwich colorimetric strategy for glycoproteins visual detection

Glycoproteins are reliable biomarkers for early cancer detection, and the timely response to these biomarkers could improve the survival rate and reduce the medical care burden. However, conventional detection technologies such as immune-based assays often need natural antibody, complex sample pretreatments and long analysis time, which failing to provide the rapid turnaround times. To address this problem, a janus gold nanoparticles nanozyme (J-GNPsNE) linked magnetic titanium dioxide molecularly imprinted particles (MTi-MIP) satellite structure immunosandwich colorimetric strategy (SS-ICS) is proposed to rapid detect glycoproteins in human serum for the first time. The unique detection mode of the strategy relies on the sandwich-like assembly of molecularly imprinted nanoparticles, glycoprotein and nanozyme. This enabled to establish a point-of-care, labor-saving, easy-to-use and noninvasive serum glycoprotein biomarkers detection tool for efficient early diagnosis of cancer. By taking transferrin (TrF) as an example, the proposed strategy can exhibits great performance with a low detection limit (0.052 μg/mL) and a short analysis time (40 min), which is much better than most of the previous methods. Our presented strategy can be easily generalized for specific detection other types of glycoproteins by changing the template, thereby facilitating the serum screening for early cancer diagnosis in non-specialized laboratory infrastructure.