紫罗兰酮
化学
桂花
类胡萝卜素
高效液相色谱法
重组DNA
萜烯
番茄红素
体外
胡萝卜素
食品科学
色谱法
生物化学
立体化学
基因
作者
Xuesong Zhang,Jianjun Pei,Linguo Zhao,Feng Tang,Xianying Fang,Jingcong Xie
出处
期刊:Journal of Molecular Catalysis B-enzymatic
[Elsevier]
日期:2016-12-01
卷期号:134: 105-114
被引量:23
标识
DOI:10.1016/j.molcatb.2016.10.003
摘要
In this study, a recombinant carotenoid cleavage dioxygenase 4 was produced from Osmanthus fragrans by E. coli under different bacterial growth conditions and used to develop a biotechnological method for preparation of natural β-ionone from β-carotene. β-ionone was analyzed by HPLC and OfCCD4 activity was measured based on concentration change of β-ionone. At pH 8.0 and 35 °C, the greatest activity of the purified recombinant protein was 14.3 U/mg and the maximum concentration of β-ionone was 71.186 mg/L within 1 h. Both the enzyme activity and the concentration of β-ionone could increase by nearly 6 times with addition of 9% Triton X-100 and 2% liquid paraffin.
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