Investigation on the determination of lignocellulosics components by NREL method

The experimental conditions of National Renewable Energy Lab (NREL) method for quantifying cellulose,hemicellulose and lignin of lignocellulosics had been investigated in detail.It was showed that the cellulose and semicellulose of corn stover could be degraded to mono-sugars quantified by HPLC under the condition of 72% concentrated sulphuric acid treating 1 h and 4% dilute acid treating 45 min with the appropriate amount of 0.5 g of sample.Meanwhile,the determination results of cellulose,hemicellulose and lignin obtained by NERL method were simultaneously compared those that by national standard method,and the results exhibited no remarkable differences.The contents of cellulose,hemicellulose and lignin of corn determined by NREL method were 33.90%,23.89 % and 15.84% respectively.