UPLC determination of ginsenoside Rg_1,ginsenoside Rb_1 and notoginsenoside R_1 in Panax Notoginseng

Objective To establish the quantitative method of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in Panax Notoginseng by ultra performance liquid chromatography(UPLC).Methods This assay was performed on ACQUITY BEH C18(2.1 mm × 50 mm,1.7 μm) column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.4 mL·min-1.And the detection wavelength was 203 nm.The column temperature was 35 ℃.The injection volume was 2 μL.Results Ginsenoside Rg1,Rb1 and notoginsenoside R1 in Panax Notoginseng had good linearity within the range of 0.116 8-1.168,0.123 6-1.236,0.030 0-0.300 μg,respectively.And their mean recovery rations were 100.1%,99.3%,99.9%with RSD of 0.4%,0.6%,1.0%(n=6).Conclusion UPLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Panax Notoginseng while reducing the solvent consumption.As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.