Inhibitory effect of Ganciclovir on human cytomegalovirus in vitro

Objective: To study the inhibitory effect of Ganciclovir(GCV) on human cytomegalovirus(HCMV) in vitro.Methods: According to viral replication cycle,three antiviral methods were designed to observe the anti-HCMV effects of Ganciclovir: Method I,Confluent cell monolayers were infected with HCMV.After some hours,it was replaced with the medium containing different served drug.Method Ⅱ,human embryo fibroblast cells were treated with the prearranged drug,Then 100 TCID50 of virus were added to these cells.Method Ⅲ,HCMV were incubated in GCV medium.Virus-drug mixed medium was added to the cells finally.By means of observing cytopathic effect and adopting MTT colorimetric assay for cell survival rate,their therapeutic index was calculated.Results: Median toxic concentration(TC50) of GCV on human embryo fibroblast cells was 1 685.23 mg/L.By method Ⅰ,the 50% inhibitory concentrations(IC50) of GCV for HCMV were 35.255 mg/L.By methodⅡand Ⅲ,GCV showed no obvious anti-virus effects.Conclusion: The above datas in vitro experimentation revealed that GCV can resist HCMV,and the inhibition effect would be strengthened with the increasing dose of GCV.It showed clear-dose connection.But GCV had no activity for guarding against human embryo fibroblast cells′ infection by HCMV in vitro.