Effects of Batroxobin on intimal proliferation of graft veins

Objective To study the effects of Batroxobin( BX) on the intimal proliferation of graft veins. Methods In this study 25 dogs were selected and evenly divided into experimental group, control group and sham operation group. In experiment and control group, a segment of auto- femoral vein were grafted into femoral artery by clean microsurgery technique. In experimental group, BX was given at the dosage of 0. 1 BU/kg, dayly×2 preoperatively, and once a day for consecutive 6 days postoperatively. Plasma NO, ET was determined in the three groups. Computer image analysis system was applied to calculate the thickness of neointima and media in the vein grafts, immunohistochemistry was used to identify PCNA and C-myc. Result The experimental group had a higher level of NO and lower level of ET compared with control group and sham operation group(P 0. 01). The area of neointima and media of graft veins in the control group was higher than that in the experimental group and sham operation group (P 0. 05). The ratio of neointima area over media area was not statistically different in experimental group compared with that in control group ( P 0. 05 ). The PCNA expression in experimental group was statistically different from that of the control group(P 0. 05). C-myc expression was higher in control group than that of experimental group(P 0. 01). Conclusion BX can inhibit intimal hyperplasia to some degree. It could be helpful for the prevention and treatment of intimal hyperplasia.