苜蓿银纹夜蛾
衣壳
生物
核质
细胞质
核型多角体病毒
病毒学
病毒
分子生物学
细胞生物学
作者
U. Bassemir,H. G. Miltenburger,P. David
摘要
Cultures of cell line IZD-MB 0503 from Mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of Autographa californica (AcNOV). At 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. Formation of virogenic stroma was observed at 7 h p.i. The first short empty capsids were seen at 10 h postinoculation (p.i.), followed by partially and completely filled nucleocapsids (11–12h p.i.) with most capsids filled at 12h and later. This suggests that nucleocapsid components, such as capsid and DNA-core, assemble in successive stages rather than simultaneously. Viral progeny, being released from the cells by budding, were observed at 13h p.i.
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