Study on Aqueous Two-phase Extraction of Teapolypheols from Tea

The method of extracting teapolypheols from tea by aqueous two-phase extraction was investigated.Experimental results indicate that using C2H5OH/K2HPO4 as aqueous two-phase extraction system,the extraction ratio can be up to 92.1 % under the conditions of 80 %C2H5OH,0.33 g/mL K2HPO4 and extraction 20 min.Thus the aqueous two-phase system may provide a new technique for separation and purification of teapolypheols.