Multienzyme‐Mimetic Activity of Gold/Cerium Oxide Nanozyme

Abstract Redox‐active nanozymes offer low‐cost controlled synthesis, high stability, and tunable catalytic properties over natural enzymes, which have attracted wide attention in the field of disease diagnosis and treatment. However, the improvement of catalytic activity remains an important challenge for nanozymes. Herein, the Au/CeO2 nanozymes is developed to achieve enhanced multiple enzyme‐mimetic activity. The Au/CeO2 nanozymes at 5% doping possess best peroxidase‐like activity with threefold higher catalytic rate than CeO2. For catalase‐mimic catalysis, the Au/CeO2 nanozymes at 5% doping also exhibited a 1.5‐fold enhanced reaction rate higher than pure CeO2. The superoxide dismutase (SOD)‐like capacity of Au/CeO2 nanozymes is proportional to Au content. The Au/CeO2 nanozymes at 10% doping show optimal SOD‐like capacity of 60.2 U mg−1. In vitro experiments validate the regulation ability of intracellular oxidative stress and inflammation. Au/CeO2 nanozymes can reduce lipopolysaccharide‐ or H2O2‐induced oxidative damage by scavenging excess ROS in nerve cell. Therefore, Au/CeO2 can be used as a promising antioxidant in disease treatment, and the study offers general guidelines for achieving enhanced biocatalytic property through atomic doping.