Heavy ion radiation induces pyroptosis in skin keratinocytes through a mitogen-activated protein kinase signaling pathway

Materials and methods Human keratinocytes (HaCaT cells) were irradiated with different doses of X-rays and 12C ions. Clonogenic survival, CCK-8 cell proliferation, and micronucleus assays were performed to assess the radiosensitivity of HaCaT cells. The pyroptosis-related, inflammation-related, and MAPK/NF-κB signaling pathway proteins were detected by Western-blotting.