FOXP3型
免疫系统
CD14型
树突状细胞
免疫学
脂质体
化学
细胞生物学
医学
生物
生物化学
作者
Noémi Nagy,Fernando Lozano Vigario,Rinske Sparrius,Toni M. M. van Capel,Ronald van Ree,Sander W. Tas,I. Jolanda M. de Vries,Teunis B. H. Geijtenbeek,Bram Slütter,Esther C. de Jong
标识
DOI:10.3389/fimmu.2023.1137538
摘要
Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted in vivo with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.
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