Protective antioxidant role of Dodoneae viscosa against oxidative stress in liver damage

Tenacity of our study is to investigate the antioxidant property of Dodonaea viscosa flower against CCl4.Dried flower of Dodonaea viscosa.To study the antioxidant potential of methanol and all its derivatives, different in vivo and in vitro antioxidant assays were performed.One being the Ethyl acetate, which was the active fraction in all assays, has expressed best results.Protective role of crude methanolic extract of Dodonaea viscosa flower was determined against oxidative stress as liver and DNA damage in rats was initiated by carbon tetrachloride.Treatment with plant extract (DVME) against oxidative damage induced by CCl4 was given on each alternate day for one month.Concentration of glutathione (GSH) and enzymes (CAT, SOD, POD) activity in liver and brain were initiated in CCl4 group in comparison to plant treated group.While for peroxidation (TBARS), lower values were noted in extract group and CCl4 group has higher values.Similarly, H2O2 and nitrite levels were significantly (p<0.005)reduced in plant treated group in comparison to CCl4 treated group.Histopathological studies showed protective role of DVME with good structural morphology at high dose (400 mg/kg), which was more operative in decreasing the toxic effects of CCl4 than lower dose (200 mg/kg).DNA damage was calculated by comet assay, in which high dose showed protective role by retaining intact DNA.These results showed that Dodonaea viscosa is medicinal plant having antioxidant potential.Besides strong antioxidant potential, it also exhibits prominent properties such as anti-microbial, anti-inflammatory and also anti-depressant activity as it possesses significant quantity of phytochemicals.