外胚层
生物
原始条纹
原肠化
胚泡
细胞生物学
胚芽层
胚胎干细胞
滋养层
内细胞团
胚胎
胚胎发生
粘合连接
诱导多能干细胞
遗传学
细胞
钙粘蛋白
胎儿
基因
胎盘
怀孕
作者
Rowan M. Karvas,Joseph E Zemke,Syed Shahzaib Ali,Eric Upton,Eshan Sane,Laura A. Fischer,Chen Dong,Kyoung Mi Park,Fei Wang,Kibeom Park,Senyue Hao,Brian Chew,Brittany Meyer,Chao Zhou,Sabine Dietmann,Thorold W. Theunissen
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2023-09-01
卷期号:30 (9): 1148-1165.e7
被引量:3
标识
DOI:10.1016/j.stem.2023.08.005
摘要
Naive human pluripotent stem cells have the remarkable ability to self-organize into blastocyst-like structures (“blastoids”) that model lineage segregation in the pre-implantation embryo. However, the extent to which blastoids can recapitulate the defining features of human post-implantation development remains unexplored. Here, we report that blastoids cultured on thick three-dimensional (3D) extracellular matrices capture hallmarks of early post-implantation development, including epiblast lumenogenesis, rapid expansion and diversification of trophoblast lineages, and robust invasion of extravillous trophoblast cells by day 14. Extended blastoid culture results in the localized activation of primitive streak marker TBXT and the emergence of embryonic germ layers by day 21. We also show that the modulation of WNT signaling alters the balance between epiblast and trophoblast fates in post-implantation blastoids. This work demonstrates that 3D-cultured blastoids offer a continuous and integrated in vitro model system of human embryonic and extraembryonic development from pre-implantation to early gastrulation stages.
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