管(容器)
重组酶聚合酶扩增
等温过程
核酸
环介导等温扩增
多重位移放大
滚动圆复制
拉曼放大
化学
单股
气泡
聚合酶链反应
材料科学
DNA聚合酶
生物化学
热力学
DNA
拉曼光谱
机械
物理
DNA提取
基因
复合材料
光学
拉曼散射
作者
Jun Guo,Yang Li,Qing Wang,Qianqian Jiang,Xinguang Zhang,Peng Zhang,Cuiping Ma,Chao Shi
摘要
Here, we established a two-stage isothermal amplification method comprising strand exchange amplification and hyperbranched rolling circle amplification, which only employed one DNA polymerase and was performed in a single closed tube at a constant temperature, providing a promising signal amplification strategy for accurate and rapid pathogen detection in the clinic.
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