The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

AbstractA genetic disorder is a disease caused by an abnormal DNA sequence, and almost half of the known pathogenic monogenetic mutations are caused by G-to-A mutation (Landrum et al., Nucleic Acids Res 44:D862–868, 2016). Adenine base editors (ABE), developed from the CRISPR, hold the great promise to mediate the A-to-G transition in genomic DNA while not inducing DNA cleavage (Gaudelli et al., Nature 551:464–471, 2017). Additionally, lipid nanoparticles (LNPs), as a non-viral delivery, are able to deliver the ABE mRNAs and gRNA to the target tissues (Newby and Liu, Mol Ther 29:3107–3124, 2021). This chapter mainly introduces the production and LNP delivery of ABE mRNA and gRNA.Key wordsABE mRNALNPsDeliveryMurine liver