生物相容性材料
移植
免疫系统
芽殖酵母
细胞
小岛
细胞生物学
癌症研究
材料科学
纳米技术
医学
化学
糖尿病
免疫学
生物
生物医学工程
内科学
生物化学
内分泌学
酿酒酵母
酵母
作者
Matthew A. Bochenek,Ben Walters,Jingping Zhang,Owen S. Fenton,Amanda Facklam,Zuzana Kroneková,Michal Pelach,Elise N. Engquist,Nayara C. Leite,Alex Morgart,Igor Lacı́k,Robert Langer,Daniel G. Anderson
出处
期刊:Small
[Wiley]
日期:2024-01-11
被引量:1
标识
DOI:10.1002/smll.202307464
摘要
Abstract The transplantation of immunoisolated stem cell derived beta cell clusters (SC‐β) has the potential to restore physiological glycemic control in patients with type I diabetes. This strategy is attractive as it uses a renewable β‐cell source without the need for systemic immune suppression. SC‐β cells have been shown to reverse diabetes in immune compromised mice when transplanted as ≈300 µm diameter clusters into sites where they can become revascularized. However, immunoisolated SC‐β clusters are not directly revascularized and rely on slower diffusion of nutrients through a membrane. It is hypothesized that smaller SC‐β cell clusters (≈150 µm diameter), more similar to islets, will perform better within immunoisolation devices due to enhanced mass transport. To test this, SC‐β cells are resized into small clusters, encapsulated in alginate spheres, and coated with a biocompatible A10 polycation coating that resists fibrosis. After transplantation into diabetic immune competent C57BL/6 mice, the “resized” SC‐β cells plus the A10 biocompatible polycation coating induced long‐term euglycemia in the mice (6 months). After retrieval, the resized A10 SC‐β cells exhibited the least amount of fibrosis and enhanced markers of β‐cell maturation. The utilization of small SC‐β cell clusters within immunoprotection devices may improve clinical translation in the future.
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