异染色质
串联重复
生物
异染色质蛋白1
构成性异染色质
遗传学
直接重复
RNA干扰
重复序列
DNA
基因组
计算生物学
基因
染色质
核糖核酸
作者
Jinxin Gao,Wenqi Sun,Jie Li,Hyoju Ban,T.L. Zhang,Jingyuan Liao,Nam-Ho Kim,Soon Hoo Lee,Qingyu Dong,Robert Madramootoo,Yong Chen,Fĕi Li
标识
DOI:10.1073/pnas.2309359120
摘要
Tandem DNA repeats are often organized into heterochromatin that is crucial for genome organization and stability. Recent studies revealed that individual repeats within tandem DNA repeats can behave very differently. How DNA repeats are assembled into distinct heterochromatin structures remains poorly understood. Here, we developed a genome-wide genetic screen using a reporter gene at different units in a repeat array. This screen led to identification of a conserved protein Rex1BD required for heterochromatin silencing. Our structural analysis revealed that Rex1BD forms a four-helix bundle structure with a distinct charged electrostatic surface. Mechanistically, Rex1BD facilitates the recruitment of Clr6 histone deacetylase (HDAC) by interacting with histones. Interestingly, Rex1BD also interacts with the 14-3-3 protein Rad25, which is responsible for recruiting the RITS (RNA-induced transcriptional silencing) complex to DNA repeats. Our results suggest that coordinated action of Rex1BD and Rad25 mediates formation of distinct heterochromatin structure at DNA repeats via linking RNAi and HDAC pathways.
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